Modifying Enzymes
Bioworld Malate Dehydrogenase, 2.5 KU
Malate DehydrogenaseSpecification:Malate Dehydrogenase, Ammonium Sulfate SuspensionUltra Pure GradeSource: Porcine heartSpecific activity (25 °C): > 1200 U/mgConcentration: > 12,000 U/mLAlanine aminotransferase: < 0.005%Aspartate aminotransferase: < 0.005%Lactate dehydrogenase: < 0.005%Unit Definition: The amount of enzyme which will convert 1 micromole of oxaloacetate and NADH per minute at 25 C in phosphate buffer at pH 7.5.
Bioworld Alkaline Phosphatase, 2500 Unit(s)
Alkaline Phosphatase1,000 UConjugation GradeAmmonium Sulfate SuspensionSource: Calf Intestine Adenosine Deaminase: <=0.001%Concentration (37 C): >7500U/mlPhosphodiesterase: <=0.001%DNase, RNase, and Protease: NoneSpecific activity (37 C, pH 10.5, DEA): >1500U/mg
Bioworld Pectinase, 10 g
Pectinase Also known as Poly-(1,4-a-D-galacturonide)glycanohydrolase. Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Bioworld Trypsin, TPCK-Treated, 100 mg
Protease which cleaves specifically at the -COOH side of arginie and lysine. TPCK-treated Trypsin is a serine endopeptidate. Trypsin is treated with TPCK to inactivate any remaining chymotryptic activity. This type of trypsin is modified by acetyltion of the sigma-amino groups of lysine residues to prevent autolysis.
Sigma Aldrich Fine Chemicals Biosciences Salt Active Nuclease, 5 KU
Salt active nuclease has been used to remove DNA during protein expression and purification.
Meridian Life Science P. vivax MSP-1, Recomb.
Plasmodium vivax (Malaria) merozoite surface protein 1 (MSP1) Recombinant
Creative Biomart Native Bacillus licheniformis Protease
AbbrProtease, Native (Bacillus licheniformis)AliasProteaseSourceBacillus licheniformisApplicationsThe product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.Product OverviewThe enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60°C.FormType VIII, lyophilized powder; Type I, aqueous solutionEnzyme Commission NumberEC 3.4.21.62
Bertin Corp User maintenance kit for Precellys 24 Touch
User maintenance kit for Precellys 24 Touch: 2 x Vacuum joint, 2 x Vacuum joint, 1 x Containment seal, 1 x Antirotation kit
Cayman Chemical ANTIBODY KU-0058948 5mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
As One International Inc Betaine Enhancer solution 5 M, 5 x 1 ml
Betaine Enhancer Solution is an excellent additive over a wide range of different templates, including GC-rich sequences and templates with a high degree of secondary structures. Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level. At the same time, Betaine has a sequence independent destabilizing effect on all DNA. The Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered. Furthermore, Betaine aids the processivity of thermostable polymerases and reduces "pauses" in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.
Worthington Biochemical Corporation DNASEI 20 MG
Deoxyribonuclease I, Chromatographically purified, Equivalent to 1X crystallized Dnase, A lyophilized powder with glycine as a stabilizer, Store at 2 to 8degreeC, Pack Size: 20mg, Source: Bovine Pancreas, min. activity: 2000U/mgKunitz dry w